Addgene: Forward and reverse genetic dissection of morphogenesis identifies filament-competent Candida auris strains.

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Teresa O'Meara
Santana et al

Forward and reverse genetic dissection of morphogenesis identifies filament-competent Candida auris strains.
Santana DJ, O'Meara TR
Nat Commun. 2021 Dec 10;12(1):7197. doi: 10.1038/s41467-021-27545-5. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
171103	pTO135_pENO1-CaCas9-tCyc1	Expresses Cas9 in Candida auris
171104	pTO136_pADH1-tRNA-Ala-gRNA-tracrRNA-HDV-tAgTEF2	Expresses sgRNA (ENO1 gRNA) in Candida auris.
171105	pTO128_pPZP-NATca	A. tumefaciens TI plasmid encoding NATca in T-DNA
177277	pTO149_RFP_CauNEO	Encodes a G418 (NEOr) selectable marker with codon-optimization for expression in CUG clade fungi. Also encodes RFP tagging of Candida auris ENO1 to measure transformation efficiency.

Plasmids from Article

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