Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

Addgene
Lack of Cas13a inhibition by anti-CRISPR proteins from Leptotrichia prophages.
Johnson MC, Hille LT, Kleinstiver BP, Meeske AJ, Bondy-Denomy J
Mol Cell. 2022 Jun 2;82(11):2161-2166.e3. doi: 10.1016/j.molcel.2022.05.002. Epub 2022 May 26. PubMed Article

Plasmids from Article

ID Plasmid Purpose
171128pCMV-T7-null (BPK1077)Plasmid encoding a pCMV-T7 with a 2 amino acid peptide prior to a stop codon.
171129pUC19-U6-LwaCas13acrRNA-BsmBIcassette (LTH151)LwaCas13a crRNA entry vector for pU6 expression (need to clone in spacer into BsmBI sites)

Antibodies from Article