pUC19-U6-LwaCas13acrRNA-BsmBIcassette (LTH151)
(Plasmid #171129)

• Purpose: LwaCas13a crRNA entry vector for pU6 expression (need to clone in spacer into BsmBI sites)
• Depositing Lab: Benjamin Kleinstiver
• Publication: Johnson et al Mol Cell. 2022 Jun 2;82(11):2161-2166.e3. doi: 10.1016/j.molcel.2022.05.002. Epub 2022 May 26.

Backbone

• Vector backbone: pUC19-U6 (derivative of BPK1520)
• Modifications to backbone: Cloned into BsmBI + HindIII digested BPK1520 (Addgene ID 65777)
• Vector type: Mammalian Expression, CRISPR

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: U6 promoter entry plasmid for LwaCas13a crRNA cloning
• Alt name: LTH151
• gRNA/shRNA sequence: entry plasmid
• Species: H. sapiens (human)
• Promoter: U6

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BsmBI (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: os280 (5'-CAGGGTTATTGTCTCATGAGCGG-3')

Resource Information

• Supplemental Documents:
  • pUC19-U6-LwaCas13acrRNA-BsmBIcassette (LTH151).gb
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://www.biorxiv.org/content/10.1101/2021.05.27.445852v1 for bioRxiv preprint.