One-step multiplex toolkit for efficient generation of conditional gene silencing human cell lines.
Yeung TK, Lau HW, Ma HT, Poon RYC
Mol Biol Cell. 2021 May 12:mbcE21020051. doi: 10.1091/mbc.E21-02-0051.
(Link opens in a new window)
PubMed
(Link opens in a new window)
Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 171677 | pUHD-SB-AID/Hyg | TRE-controlled AID-fusion sleeping beauty transposon vector with selection marker (hygromycin) |
| 171678 | pUHD-SB-AID/Bla | TRE-controlled AID-fusion sleeping beauty transposon vector with selection marker (blasticidin) |
| 171679 | pUHD-SB-mAID/Hyg | TRE-controlled mAID-fusion sleeping beauty transposon vector with selection marker (hygromycin) |
| 171680 | pUHD-SB-mAID/Bla | TRE-controlled mAID-fusion sleeping beauty transposon vector with selection marker (blasticidin) |
| 171681 | pUHD-SB-mAID/Zeo | TRE-controlled mAID-fusion sleeping beauty transposon vector with selection marker (zeocin) |
| 171682 | pSBbi-TIR1/Pur | constitutive expression of TIR1-myc in sleeping beauty transposon vector with selection marker (puromycin) |
| 171683 | pSBbi-TIR1-tTA/Pur | constitutive expression of TIR1-myc and tTA in sleeping beauty transposon vector with selection marker (puromycin) |
| 171684 | pSBbi-TIR1-tTA/Neo | constitutive expression of TIR1-myc and tTA in sleeping beauty transposon vector with selection marker (G418) |
| 171685 | pSBbi-TIR1-tTA/Zeo | constitutive expression of TIR1-myc and tTA in sleeping beauty transposon vector with selection marker (zeocin) |