SLFN2 protection of tRNAs from stress-induced cleavage is essential for T cell-mediated immunity.
Yue T, Zhan X, Zhang D, Jain R, Wang KW, Choi JH, Misawa T, Su L, Quan J, Hildebrand S, Xu D, Li X, Turer E, Sun L, Moresco EMY, Beutler B
Science. 2021 May 14;372(6543). pii: 372/6543/eaba4220. doi: 10.1126/science.aba4220. PubMed Article
Yue T, Zhan X, Zhang D, Jain R, Wang KW, Choi JH, Misawa T, Su L, Quan J, Hildebrand S, Xu D, Li X, Turer E, Sun L, Moresco EMY, Beutler B
Science. 2021 May 14;372(6543). pii: 372/6543/eaba4220. doi: 10.1126/science.aba4220. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 174307 | pSin-GFP-Fg | Lentiviral expression of GFP |
| 174317 | pBS-tRNA-Gly | For in vitro transcription of mouse tRNAs |
| 174318 | pBS-tRNA-Ser | For in vitro transcription of mouse tRNAs |
| 174319 | pEZT-GST-SLFN2 | Expression of recombinant GST-SLFN2 |
| 174320 | pEZT-GST-SLFN2-T1 | Expression of recombinant GST-SLFN2-R67A, K68A, N71A |
| 174321 | pEZT-GST-SLFN2-T2 | Expression of recombinant GST-SLFN2-K242A, K249A, Q301A |
| 174322 | pMal-SLFN2 | Bacterial expression of MBP-tagged mouse SLFN2 |