Addgene: Domesticating a food spoilage yeast into an organic acid-tolerant metabolic engineering host: Lactic acid production by engineered Zygosaccharomyces bailii.

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Yong-Su Jin
Kuanyshev et al

Domesticating a food spoilage yeast into an organic acid-tolerant metabolic engineering host: Lactic acid production by engineered Zygosaccharomyces bailii.
Kuanyshev N, Rao CV, Dien B, Jin YS
Biotechnol Bioeng. 2021 Jan;118(1):372-382. doi: 10.1002/bit.27576. Epub 2020 Oct 8. PubMed Article

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ID	Plasmid	Purpose
200169	pCast	E. coli/Yeast shuttle vector carrying CloNAT marker and Spcas9 allowing cloning of gRNA flanked by tRNA promoter and HDV ribozyme

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