pCast
(Plasmid
#200169)
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Purpose(Empty Backbone) E. coli/Yeast shuttle vector carrying CloNAT marker and Spcas9 allowing cloning of gRNA flanked by tRNA promoter and HDV ribozyme
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 200169 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonenot applicable
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Vector typeYeast Expression, CRISPR
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Selectable markersNourseothricin (clonNAT)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Cloning Information
- Cloning method Restriction Enzyme
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Addgene identified a duplication of the natMX6 feature relative to the original reference sequence from the depositing lab. The depositors have confirmed they have also observed this duplication and that the plasmid still functions as described in their associated publication.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCast was a gift from Yong-Su Jin (Addgene plasmid # 200169 ; http://n2t.net/addgene:200169 ; RRID:Addgene_200169) -
For your References section:
Domesticating a food spoilage yeast into an organic acid-tolerant metabolic engineering host: Lactic acid production by engineered Zygosaccharomyces bailii. Kuanyshev N, Rao CV, Dien B, Jin YS. Biotechnol Bioeng. 2021 Jan;118(1):372-382. doi: 10.1002/bit.27576. Epub 2020 Oct 8. 10.1002/bit.27576 PubMed 33030791
