Genome-wide interrogation of gene functions through base editor screens empowered by barcoded sgRNAs.
Xu P, Liu Z, Liu Y, Ma H, Xu Y, Bao Y, Zhu S, Cao Z, Wu Z, Zhou Z, Wei W
Nat Biotechnol. 2021 Jun 21. pii: 10.1038/s41587-021-00944-1. doi: 10.1038/s41587-021-00944-1.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 174163 | BARBEKO sgRNA library | Cytosine base editors for genome-scale knockout screens in human cells. Targets gene start codons, splice sites, or introduces premature termination codons. |
| 174164 | pLenti-AncBE4max | This is an AncBE4max (a cytosine base editor) expressing plasmid built on the 3rd generation system of lentiviral vector. It can be used for stable expression of AncBE4max in cells. |