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Proteomics of protein trafficking by in vivo tissue-specific labeling.
Droujinine IA, Meyer AS, Wang D, Udeshi ND, Hu Y, Rocco D, McMahon JA, Yang R, Guo J, Mu L, Carey DK, Svinkina T, Zeng R, Branon T, Tabatabai A, Bosch JA, Asara JM, Ting AY, Carr SA, McMahon AP, Perrimon N
Nat Commun. 2021 Apr 22;12(1):2382. doi: 10.1038/s41467-021-22599-x.
PubMed Article

Plasmids from Article

ID Plasmid Purpose
173814pEntr_Myc-BirA-G3-ERPromiscuous biotin ligase (BirA-G3) targeted to ER lumen in Gateway entry vector. N-terminal secretion signal from Drosophila BiP. C-terminal KDEL. Myc tag inserted after sig. seq. cleavage site
173815pUAS-Myc-BirA-G3-ERPromiscuous biotin ligase (BirA-G3) targeted to ER lumen expressed from UAS enhancer. N-terminal secretion signal from Drosophila BiP. C-terminal KDEL. Myc tag inserted after sig. seq. cleavage site

Antibodies from Article