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The AMBRA1 E3 ligase adaptor regulates the stability of cyclin D.
Chaikovsky AC, Li C, Jeng EE, Loebell S, Lee MC, Murray CW, Cheng R, Demeter J, Swaney DL, Chen SH, Newton BW, Johnson JR, Drainas AP, Shue YT, Seoane JA, Srinivasan P, He A, Yoshida A, Hipkins SQ, McCrea E, Poltorack CD, Krogan NJ, Diehl JA, Kong C, Jackson PK, Curtis C, Petrov DA, Bassik MC, Winslow MM, Sage J
Nature. 2021 Apr;592(7856):794-798. doi: 10.1038/s41586-021-03474-7. Epub 2021 Apr 14.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
174142pKHH030_sgCtrl#1Lentiviral vector expressing a control sgRNA that targets a safe harbor site
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174143pKHH030_sgCtrl#2Lentiviral vector expressing a control sgRNA that targets a safe harbor site
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174144pKHH030_sgRB1#1Lentiviral vector expressing a sgRNA against the human RB1 gene
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174145pKHH030_sgRB1#2Lentiviral vector expressing a sgRNA against the human RB1 gene
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174146pKHH030_sgAMBRA1#1Lentiviral vector expressing a sgRNA against the human AMBRA1 gene
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174147pKHH030_sgAMBRA1#2Lentiviral vector expressing a sgRNA against the human AMBRA1 gene
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174148lentiCRISPRv2_sgCtrl#1Lentiviral vector expressing Cas9 and a control sgRNA that targets a safe harbor site
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174149lentiCRISPRv2_sgCtrl#2Lentiviral vector expressing Cas9 and a control sgRNA that targets a safe harbor site
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174150lentiCRISPRv2_RB1#1Lentiviral vector expressing Cas9 and a sgRNA against the human RB1 gene
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174151lentiCRISPRv2_RB1#2Lentiviral vector expressing Cas9 and a sgRNA against the human RB1 gene
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174152lentiCRISPRv2_AMBRA1#1Lentiviral vector expressing Cas9 and a sgRNA against the human AMBRA1 gene
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174153lentiCRISPRv2_AMBRA1#2Lentiviral vector expressing Cas9 and a sgRNA against the human AMBRA1 gene
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174154pLV-hCCND1-HALentiviral vector expressing HA-tagged human cyclin D1
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174155pLV-hCCND1-T286A-HALentiviral vector expressing HA-tagged human cyclin D1 with T286A mutation
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174156pLV-hCCND1-T2A-hCCND2-P2A-hCCND3Lentiviral vector expressing all three D-type cyclins (cyclin D1, cyclin D2, cyclin D3) from a single promoter
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174157pcDNA4-AMBRA1-3xFLAGVector for expression of 3xFLAG-tagged wild-type AMBRA1
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174158pcDNA4-deltaH-AMBRA1-3xFLAGVector for expression of 3xFLAG-tagged truncated AMBRA1 that cannot bind DDB1 (aa 1-34 deleted)
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174159pPB-AMBRA1-3xFLAGPiggyBac vector to stably express 3xFLAG-tagged AMBRA1
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174160pPB-AMBRA1-501*-3xFLAGPiggyBac vector to stably express 3xFLAG-tagged AMBRA1 truncated at amino acid 501
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174161pPB-AMBRA1-217*-3xFLAGPiggyBac vector to stably express 3xFLAG-tagged AMBRA1 truncated at amino acid 217
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Recombinant Antibodies from Article