Genetic Encoding of Cyanopyridylalanine for In-Cell Protein Macrocyclization by the Nitrile-Aminothiol Click Reaction.
Abdelkader EH, Qianzhu H, George J, Frkic RL, Jackson CJ, Nitsche C, Otting G, Huber T
Angew Chem Int Ed Engl. 2022 Jan 31. doi: 10.1002/anie.202114154. PubMed Article
Abdelkader EH, Qianzhu H, George J, Frkic RL, Jackson CJ, Nitsche C, Otting G, Huber T
Angew Chem Int Ed Engl. 2022 Jan 31. doi: 10.1002/anie.202114154. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 174718 | pRSF-G1mCNPRS | tRNA synthetase/tRNA pair for the in vivo incorporation of L-3-(2-cyano-4-pyridyl)alanine (mCNP), into proteins in E. coli in response to the amber (TAG) codon |
| 174719 | pRSF-G1pCNPRS | tRNA synthetase/tRNA pair for the in vivo incorporation of L-3-(2-cyano-5-pyridyl)alanine (pCNP), into proteins in E. coli in response to the amber (TAG) codon |