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Exploiting Polyploidy for Markerless and Plasmid-Free Genome Engineering in Cyanobacteria.
Jones CM, Parrish S, Nielsen DR
ACS Synth Biol. 2021 Sep 17;10(9):2371-2382. doi: 10.1021/acssynbio.1c00269. Epub 2021 Aug 17. PubMed Article

Plasmids from Article

ID Plasmid Purpose
175052pCJ111rbcLXS7002 integrative plasmid expresses CRE recombinase as a translational fusion to rbcL
175054pCJ155psbEFLJ integrative plasmid expresses CRE recombinase from the rbcL promoter uses zeocin to integrate
175055pCJ162lox66-72 flanked gentamycin resistance cassette integrates into aquI integration site
175056pCJ163rox flanked gentamycin resistance cassette integrates into A2542 integration site
175057pCJ164rbcLXS7002 integrative plasmid expresses DRE recombinase as a translational fusion to rbcL
175058pZD-CJ1111rbcLXS6803 integrative plasmid expresses CRE recombinase as a translational fusion to rbcL6803

Antibodies from Article