FAS2FURIOUS: Moderate-Throughput Secreted Expression of Difficult Recombinant Proteins in Drosophila S2 Cells.
Coker JA, Katis VL, Fairhead M, Schwenzer A, Clemmensen SB, Frandsen BU, de Jongh WA, Gileadi O, Burgess-Brown NA, Marsden BD, Midwood KS, Yue WW
Front Bioeng Biotechnol. 2022 May 5;10:871933. doi: 10.3389/fbioe.2022.871933. eCollection 2022. PubMed Article
Coker JA, Katis VL, Fairhead M, Schwenzer A, Clemmensen SB, Frandsen BU, de Jongh WA, Gileadi O, Burgess-Brown NA, Marsden BD, Midwood KS, Yue WW
Front Bioeng Biotechnol. 2022 May 5;10:871933. doi: 10.3389/fbioe.2022.871933. eCollection 2022. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 175360 | pExpreS2-1 | ZeoR backbone for recombinant protein overexpression with Drosophila ExpreS2 Platform |
| 175362 | pExpreS2-2 | G418R backbone for recombinant protein overexpression with Drosophila ExpreS2 Platform |
| 175363 | pExpreS2-1-GFP | ZeoR overexpression of cytosolic GFP in Drosophila ExpreS2ion cells |
| 175443 | pExpreS2-1-C3-PA-10H-SIII | HiFi-compatible destination vector for secreted overexpression of 3C-cleavable, C-terminal Protein A-10His-TwinStrep tagged proteins with ExpreS2 Platform |
| 175444 | pExpreS2-1-C3-10H-SIII | HiFi-compatible destination vector for secreted overexpression of 3C-cleavable, C-terminal 10His-TwinStrep tagged proteins with ExpreS2 Platform |
| 175445 | pExpreS2-1-CR-PA | HiFi-compatible destination vector for secreted overexpression of thrombin-cleavable, C-terminal Protein A tagged proteins with ExpreS2 Platform |
| 175446 | pExpreS2-1-C3-PA | HiFi-compatible destination vector for secreted overexpression of 3C-cleavable, C-terminal Protein A tagged proteins with ExpreS2 Platform |
| 175447 | pExpreS2-1-CR-PA-10H-SIII | HiFi-compatible destination vector for secreted overexpression of thrombin-cleavable, C-terminal Protein A-10His-TwinStrep tagged proteins with ExpreS2 Platform |