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Tension promotes kinetochore-microtubule release by Aurora B kinase.
Chen GY, Renda F, Zhang H, Gokden A, Wu DZ, Chenoweth DM, Khodjakov A, Lampson MA
J Cell Biol. 2021 Jun 7;220(6). pii: 212027. doi: 10.1083/jcb.202007030. PubMed Article

Plasmids from Article

ID Plasmid Purpose
176201CAG.mCherry-eDHFRNegative control of targeted optogenetic manipulation using TMP-Halo dimerization system
176202CAG.mCherry-INBox-eDHFRSingle INBox (INCENP 819-918) recruitment responsible for targeted Aurora B activation and recruitment using TMP-Halo dimerization system
176203CAG.INBox-mCherry-INBox-eDHFRDouble INBox (INCENP 819-918) recruitment responsible for targeted Aurora B activation and recruitment using TMP-Halo dimerization system
176204CAG.MCAK-mCherry-eDHFRMCAK (179-583) recruitment responsible for targeted microtubule depolymerization using TMP-Halo dimerization system
176205CAG.CenpB(FL)-GFP-HaloCentromeric anchor for TMP-Halo dimerization system
176206CAG.3xHalo-GFP-SPC25Kinetochore anchor for TMP-Halo dimerization system
176207CAG.mCherry-INBox(AAA)-eDHFRINBox TSS(892-894)-mutant replaced by AAA responsible for targeted Aurora B recruitment without activation using TMP-Halo dimerization system

Antibodies from Article