CAG.mCherry-eDHFR
(Plasmid
#176201)
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PurposeNegative control of targeted optogenetic manipulation using TMP-Halo dimerization system
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Depositing Lab
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Depositing OrganizationUniversity of Pennsylvania
Located in United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 176201 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 176201-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 176201-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepEM791
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Backbone manufacturerDr. Eugene Makeyev
- Backbone size w/o insert (bp) -1
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Vector typeMammalian Expression
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemCherry-eDHFR
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SpeciesE. coli
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please note that there are minor differences between the Addgene NGS result and the depositor's sequence. These differences do not alter plasmid function.
DNA (Catalog # 176201-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 176201-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
CAG.mCherry-eDHFR was a gift from Michael Lampson (Addgene plasmid # 176201 ; http://n2t.net/addgene:176201 ; RRID:Addgene_176201) -
For your References section:
Tension promotes kinetochore-microtubule release by Aurora B kinase. Chen GY, Renda F, Zhang H, Gokden A, Wu DZ, Chenoweth DM, Khodjakov A, Lampson MA. J Cell Biol. 2021 Jun 7;220(6). pii: 212027. doi: 10.1083/jcb.202007030. 10.1083/jcb.202007030 PubMed 33904910
Map uploaded by the depositor.
