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A cell-free nanobody engineering platform rapidly generates SARS-CoV-2 neutralizing nanobodies.
Chen X, Gentili M, Hacohen N, Regev A
Nat Commun. 2021 Sep 17;12(1):5506. doi: 10.1038/s41467-021-25777-z.
PubMed Article

Plasmids from Article

ID Plasmid Purpose
176208pBx1-VHH template1-3XMyc-SpacerPCR template for generating CeVICA VHH/nanobody library
176209pBx1-VHH template2-3XMyc-SpacerPCR template for generating CeVICA VHH/nanobody library
176210pBx1-VHH template3-3XMyc-SpacerPCR template for generating CeVICA VHH/nanobody library
176211pETx-SR6c3Bacterial expression of the SR6c3 nanobody targeting the receptor binding domain of SARS-CoV-2 spike protein
176212pETx-SR6v15Bacterial expression of the SR6v15 nanobody targeting the receptor binding domain of SARS-CoV-2 spike protein
176213pETx-SR6v15.dBacterial expression of the SR6v15.d targeting the receptor binding domain of SARS-CoV-2 spike protein
176214pETx-SR6v15.tBacterial expression of the SR6v15.t targeting the receptor binding domain of SARS-CoV-2 spike protein

Antibodies from Article