Direct delivery and fast-treated Agrobacterium co-culture (Fast-TrACC) plant transformation methods for Nicotiana benthamiana.
Cody JP, Maher MF, Nasti RA, Starker CG, Chamness JC, Voytas DF
Nat Protoc. 2022 Oct 17. pii: 10.1038/s41596-022-00749-9. doi: 10.1038/s41596-022-00749-9. PubMed Article
Cody JP, Maher MF, Nasti RA, Starker CG, Chamness JC, Voytas DF
Nat Protoc. 2022 Oct 17. pii: 10.1038/s41596-022-00749-9. doi: 10.1038/s41596-022-00749-9. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 176863 | pTAG1 | First sgRNA in a tRNA separated array of sgRNAs |
| 176864 | pTAG2 | First sgRNA in a tRNA separated array of sgRNAs |
| 176865 | pTAG2E | First sgRNA in a tRNA separated array of sgRNAs |
| 176866 | pTAG3 | First sgRNA in a tRNA separated array of sgRNAs |
| 176867 | pTAG4 | First sgRNA in a tRNA separated array of sgRNAs |
| 176868 | pTAG4E | First sgRNA in a tRNA separated array of sgRNAs |
| 176869 | pTAG5 | First sgRNA in a tRNA separated array of sgRNAs |
| 176870 | pTAG6E | First sgRNA in a tRNA separated array of sgRNAs |
| 176871 | pMC-1-AtUbi10-6 | MoClo Level 0 plasmid, contains AtUbi1 promoter |
| 176872 | pMC-1-CmYLCV-6 | MoClo Level 0 plasmid, contains CmYLCV-6 promoter |
| 176873 | pMOD_A4800EC | Empty backbone for Golden Gate cloning into Cermak, et al., 2017 Toolkit |
| 176874 | pMOD_B4800EC | Empty backbone for Golden Gate cloning into Cermak, et al., 2017 Toolkit |
| 176875 | pMOD_C'4800EC | Empty backbone for Golden Gate cloning into Cermak, et al., 2017 Toolkit |
| 176876 | pMOD_D4800EC | Empty backbone for Golden Gate cloning into Cermak, et al., 2017 Toolkit |
| 176877 | pMOD_A5801 | High constitutive expression of Firefly Luciferase in plants |
| 176878 | pMOD_A5803 | High constitutive expression of Firefly Luciferase in plants |
| 176879 | pMOD_C'5802 | High constitutive expression of Firefly Luciferase in plants |
| 176880 | pMOD_D5802 | High constitutive expression of Firefly Luciferase in plants |
| 176881 | pMC-9-35S-11 | MoClo Level 0 plasmid, contains 35S |
| 176882 | pMC-9-HSP-11 | MoClo Level 0 plasmid, contains HSP |
| 176883 | pJC225 | MoClo Level1 plasmid, allows transition into Cermak, et al., 2017 GG cloning system |
| 176884 | pRN119 | Encodes STM and WUS2 for ectopic meristem induction in plants |