pTAG1
(Plasmid
#176863)
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PurposeFirst sgRNA in a tRNA separated array of sgRNAs
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 176863 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepAGM9121
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Vector typeCRISPR
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namesgRNA_1
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Alt nameLacZ
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gRNA/shRNA sequenceunknown
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SpeciesSynthetic
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MutationWT
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 3′ cloning site Unknown (unknown if destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
MoClo compatible
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pTAG1 was a gift from Daniel Voytas (Addgene plasmid # 176863 ; http://n2t.net/addgene:176863 ; RRID:Addgene_176863) -
For your References section:
Direct delivery and fast-treated Agrobacterium co-culture (Fast-TrACC) plant transformation methods for Nicotiana benthamiana. Cody JP, Maher MF, Nasti RA, Starker CG, Chamness JC, Voytas DF. Nat Protoc. 2022 Oct 17. pii: 10.1038/s41596-022-00749-9. doi: 10.1038/s41596-022-00749-9. 10.1038/s41596-022-00749-9 PubMed 36253612
