An Ultra-Sensitive Step-Function Opsin for Minimally Invasive Optogenetic Stimulation in Mice and Macaques.
Gong X, Mendoza-Halliday D, Ting JT, Kaiser T, Sun X, Bastos AM, Wimmer RD, Guo B, Chen Q, Zhou Y, Pruner M, Wu CW, Park D, Deisseroth K, Barak B, Boyden ES, Miller EK, Halassa MM, Fu Z, Bi G, Desimone R, Feng G
Neuron. 2020 Jul 8;107(1):197. doi: 10.1016/j.neuron.2020.06.018. PubMed Article
Gong X, Mendoza-Halliday D, Ting JT, Kaiser T, Sun X, Bastos AM, Wimmer RD, Guo B, Chen Q, Zhou Y, Pruner M, Wu CW, Park D, Deisseroth K, Barak B, Boyden ES, Miller EK, Halassa MM, Fu Z, Bi G, Desimone R, Feng G
Neuron. 2020 Jul 8;107(1):197. doi: 10.1016/j.neuron.2020.06.018. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 153019 | pAAV-hSyn-SOUL-P2A-tdTomato | Step-function opsin with ultra-high light sensitivity (SOUL). Capable to be used for noninvasive stimulation in mice and minimally invasive stimulation in macaque. |
| 177576 | pAAV-hSyn-SOUL-P2A-TdTomato-WPRE-hGH-polyA | Expresses SOUL in neuronal cell types |
| 177577 | pAAV-EF1a-FLEX-SOUL-P2A-tdTomato-WPRE-BGHpA | Expresses SOUL in Cre-positive cells |