Spatial CRISPR genomics identifies regulators of the tumor microenvironment.
Dhainaut M, Rose SA, Akturk G, Wroblewska A, Nielsen SR, Park ES, Buckup M, Roudko V, Pia L, Sweeney R, Le Berichel J, Wilk CM, Bektesevic A, Lee BH, Bhardwaj N, Rahman AH, Baccarini A, Gnjatic S, Pe'er D, Merad M, Brown BD
Cell. 2022 Mar 10. pii: S0092-8674(22)00195-7. doi: 10.1016/j.cell.2022.02.015. PubMed Article
Dhainaut M, Rose SA, Akturk G, Wroblewska A, Nielsen SR, Park ES, Buckup M, Roudko V, Pia L, Sweeney R, Le Berichel J, Wilk CM, Bektesevic A, Lee BH, Bhardwaj N, Rahman AH, Baccarini A, Gnjatic S, Pe'er D, Merad M, Brown BD
Cell. 2022 Mar 10. pii: S0092-8674(22)00195-7. doi: 10.1016/j.cell.2022.02.015. PubMed Article
These materials are included in the Pro-Code Vector Kit (#1000000197).
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 178209 | pLKO_AU1.C.HA_mCherry-NLS | Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens. |
| 178210 | pLKO_AU1.C.HSV_mCherry-NLS | Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens. |
| 178211 | pLKO_Ollas.S.V5_mCherry-NLS | Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens. |
| 178212 | pLKO_AU1.C.Ollas_mCherry-NLS | Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens. |
| 178213 | pLKO_AU1.C.S_mCherry-NLS | Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens. |
| 178214 | pLKO_AU1.C.VSVg_mCherry-NLS | Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens. |
| 178215 | pLKO_AU1.C.V5_mCherry-NLS | Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens. |
| 178216 | pLKO_AU1.HA.HSV_mCherry-NLS | Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens. |
| 178217 | pLKO_AU1.HA.NWS_mCherry-NLS | Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens. |
| 178218 | pLKO_AU1.HA.Ollas_mCherry-NLS | Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens. |
| 178219 | pLKO_AU1.HA.S_mCherry-NLS | Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens. |
| 178220 | pLKO_AU1.HA.VSVg_mCherry-NLS | Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens. |
| 178221 | pLKO_AU1.HA.V5_mCherry-NLS | Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens. |
| 178222 | pLKO_AU1.HSV.NWS_mCherry-NLS | Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens. |
| 178223 | pLKO_AU1.HSV.Ollas_mCherry-NLS | Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens. |
| 178224 | pLKO_AU1.HSV.S_mCherry-NLS | Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens. |
| 178225 | pLKO_AU1.HSV.VSVg_mCherry-NLS | Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens. |
| 178226 | pLKO_AU1.HSV.V5_mCherry-NLS | Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens. |
| 178227 | pLKO_AU1.NWS.Ollas_mCherry-NLS | Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens. |
| 178228 | pLKO_AU1.NWS.S_mCherry-NLS | Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens. |
| 178229 | pLKO_AU1.NWS.VSVg_mCherry-NLS | Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens. |
| 178230 | pLKO_AU1.NWS.V5_mCherry-NLS | Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens. |
| 178231 | pLKO_AU1.Ollas.S_mCherry-NLS | Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens. |
| 178232 | pLKO_AU1.Ollas.VSVg_mCherry-NLS | Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens. |
| 178233 | pLKO_AU1.Ollas.V5_mCherry-NLS | Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens. |
| 178234 | pLKO_AU1.S.VSVg_mCherry-NLS | Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens. |
| 178235 | pLKO_AU1.S.V5_mCherry-NLS | Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens. |
| 178236 | pLKO_AU1.VSVg.V5_mCherry-NLS | Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens. |
| 178237 | pLKO_C.HA.HSV_mCherry-NLS | Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens. |
| 178238 | pLKO_C.HA.NWS_mCherry-NLS | Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens. |
| 178239 | pLKO_C.HA.Ollas_mCherry-NLS | Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens. |
| 178240 | pLKO_C.HA.S_mCherry-NLS | Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens. |
| 178241 | pLKO_C.HA.VSVg_mCherry-NLS | Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens. |
| 178242 | pLKO_C.HA.V5_mCherry-NLS | Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens. |
| 178243 | pLKO_C.HSV.NWS_mCherry-NLS | Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens. |
| 178244 | pLKO_C.HSV.Ollas_mCherry-NLS | Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens. |
| 178245 | pLKO_C.HSV.S_mCherry-NLS | Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens. |
| 178246 | pLKO_C.HSV.VSVg_mCherry-NLS | Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens. |
| 178247 | pLKO_C.HSV.V5_mCherry-NLS | Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens. |
| 178248 | pLKO_C.NWS.Ollas_mCherry-NLS | Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens. |
| 178249 | pLKO_C.NWS.S_mCherry-NLS | Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens. |
| 178250 | pLKO_C.NWS.VSVg_mCherry-NLS | Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens. |
| 178251 | pLKO_C.NWS.V5_mCherry-NLS | Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens. |
| 178252 | pLKO_C.Ollas.S_mCherry-NLS | Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens. |
| 178253 | pLKO_C.Ollas.VSVg_mCherry-NLS | Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens. |
| 178254 | pLKO_C.Ollas.V5_mCherry-NLS | Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens. |
| 178255 | pLKO_C.S.VSVg_mCherry-NLS | Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens. |
| 178256 | pLKO_C.S.V5_mCherry-NLS | Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens. |
| 178257 | pLKO_C.VSVg.V5_mCherry-NLS | Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens. |
| 178258 | pLKO_HA.HSV.NWS_mCherry-NLS | Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens. |
| 178259 | pLKO_HA.HSV.Ollas_mCherry-NLS | Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens. |
| 178260 | pLKO_HA.HSV.S_mCherry-NLS | Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens. |
| 178261 | pLKO_HA.HSV.VSVg_mCherry-NLS | Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens. |
| 178262 | pLKO_HA.HSV.V5_mCherry-NLS | Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens. |
| 178263 | pLKO_HA.NWS.Ollas_mCherry-NLS | Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens. |
| 178264 | pLKO_HA.NWS.S_mCherry-NLS | Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens. |
| 178265 | pLKO_HA.NWS.VSVg_mCherry-NLS | Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens. |
| 178266 | pLKO_HA.Ollas.S_mCherry-NLS | Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens. |
| 178267 | pLKO_HA.Ollas.VSVg_mCherry-NLS | Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens. |
| 178268 | pLKO_HA.Ollas.V5_mCherry-NLS | Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens. |
| 178269 | pLKO_HA.S.VSVg_mCherry-NLS | Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens. |
| 178270 | pLKO_HA.S.V5_mCherry-NLS | Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens. |
| 178271 | pLKO_HA.VSVg.V5_mCherry-NLS | Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens. |
| 178272 | pLKO_HSV.NWS.Ollas_mCherry-NLS | Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens. |
| 178273 | pLKO_HSV.NWS.S_mCherry-NLS | Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens. |
| 178274 | pLKO_HSV.NWS.VSVg_mCherry-NLS | Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens. |
| 178275 | pLKO_HSV.NWS.V5_mCherry-NLS | Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens. |
| 178276 | pLKO_HSV.Ollas.S_mCherry-NLS | Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens. |
| 178277 | pLKO_HSV.Ollas.V5_mCherry-NLS | Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens. |
| 178278 | pLKO_HSV.S.V5_mCherry-NLS | Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens. |
| 178279 | pLKO_NWS.Ollas.S_mCherry-NLS | Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens. |
| 178280 | pLKO_NWS.Ollas.V5_mCherry-NLS | Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens. |
| 178281 | pLKO_NWS.S.V5_mCherry-NLS | Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens. |
| 178282 | pLKO_AU1.C.NWS_mCherry-NLS | Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens. |
| 178283 | pLKO_NWS.HA.V5_mCherry-NLS | Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens. |