Revisiting astrocyte to neuron conversion with lineage tracing in vivo.
Wang LL, Serrano C, Zhong X, Ma S, Zou Y, Zhang CL
Cell. 2021 Oct 14;184(21):5465-5481.e16. doi: 10.1016/j.cell.2021.09.005. Epub 2021 Sep 27.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 178580 | AAV-hGFAP-GFP | Expression of GFP under the human GFAP promoter |
| 178581 | AAV-hGFAP-mCherry | Expression of mCherry under the human GFAP promoter |
| 178582 | AAV-hGFAP-NEUROD1-T2A-mCherry | Expression of NEUROD1 and mCherry under the human GFAP promoter |
| 178583 | AAV-CAG-FLEX-mCherry | Cre-dependent expression of mCherry under the constitutive promoter |
| 178584 | AAV-CAG-FLEX-NEUROD1-T2A-mCherry | Cre-dependent expression of NEUROD1 and mCherry under the constitutive promoter |
| 178585 | AAV-CAG-FLEX-HA-DLX2-T2A-mCherry | Cre-dependent expression of HA-DLX2 and mCherry under the constitutive promoter |
| 178586 | AAV-hGFAP-ND1-R111L-T2A-mCherry | Expression of NEUROD1-R111L and mCherry under the human GFAP promoter |
| 178587 | AAV-hGFAP-ND1-K139D-T2A-mCherry | Expression of NEUROD1-K139D and mCherry under the human GFAP promoter |
| 178588 | AAV-hGFAP-mCherry-shLuci | Expression of mCherry and a shRNA against luciferase under the human GFAP promoter |
| 178589 | AAV-hGFAP-mCherry-shPtbp1 | Expression of mCherry and a shRNA against Ptbp1 under the human GFAP promoter |
| 178590 | AAV-CAG-LSL-mCherry-shLuci | Cre-dependent expression of mCherry and a shRNA against luciferase under the constitutive promoter |
| 178591 | AAV-CAG-LSL-mCherry-shPtbp1 | Cre-dependent expression of mCherry and a shRNA against Ptbp1 under the constitutive promoter |