Biophysical characterization and a roadmap towards the NMR solution structure of G0S2, a key enzyme in non-alcoholic fatty liver disease.
Moran MW, Ramirez EP, Zook JD, Saarinen AM, Baravati B, Goode MR, Laloudakis V, Kaschner EK, Olson TL, Craciunescu FM, Hansen DT, Liu J, Fromme P
PLoS One. 2021 Jul 14;16(7):e0249164. doi: 10.1371/journal.pone.0249164. eCollection 2021. PubMed Article
Moran MW, Ramirez EP, Zook JD, Saarinen AM, Baravati B, Goode MR, Laloudakis V, Kaschner EK, Olson TL, Craciunescu FM, Hansen DT, Liu J, Fromme P
PLoS One. 2021 Jul 14;16(7):e0249164. doi: 10.1371/journal.pone.0249164. eCollection 2021. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 179293 | pHis6-SUMO-g0s2 | E. coli expression plasmid (T7 promoter) for full-length human G0S2 with N-terminal His6 + SUMO + TEV protease cleavage site |
| 179294 | MBP-g0s2 | E. coli expression plasmid (Ptac promoter) for full-length human G0S2 with N-terminal mature maltose binding protein + enterokinase cleavage site |