MBP-g0s2
(Plasmid #179294)

• Purpose: E. coli expression plasmid (Ptac promoter) for full-length human G0S2 with N-terminal mature maltose binding protein + enterokinase cleavage site
• Depositing Lab: Debra Hansen
• Publication: Moran et al PLoS One. 2021 Jul 14;16(7):e0249164. doi: 10.1371/journal.pone.0249164. eCollection 2021.

Backbone

• Vector backbone: pMAL-c5E
• Backbone manufacturer: New England Biolabs
• Backbone size w/o insert (bp): 5676
• Total vector size (bp): 5994
• Modifications to backbone: none
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: G0S2
• Alt name: G0/G1 switch regulatory protein 2
• Species: H. sapiens (human)
• Insert Size (bp): 318
• Mutation: full-length G0S2, residues 1-103 from accession number NP_056529.1; optimized DNA sequence
• GenBank ID: NP_056529.1
• Promoter: Ptac
• Tag / Fusion Protein:
  • mature maltose binding protein + enterokinase cleavage site (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (not destroyed)
• 3′ cloning site: NcoI (not destroyed)
• 5′ sequencing primer: MBP-F, GATGAAGCCCTGAAAGACGCGCAG
• 3′ sequencing primer: pMALREV, TGTCCTACTCAGGAGAGCGTTCAC

Resource Information

• Supplemental Documents:
  • MBP-g0s2.gb
• A portion of this plasmid was derived from a plasmid made by: The insert had been ordered from GenScript as an E. coli expression-optimized synthetic DNA.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Protein sequence is the mature maltose binding protein (identical to aa 1-367 of accession no. 6K7E_A) + NSSSNNNNNNNNNNLG + enterokinase cleavage site (DDDDK*; * = cleavage site) + VPH + human G0S2 (G0/G1 switch protein 2; accession number NP_056529.1) + L.
Confers ampicillin-resistance.
This plasmid has been referred to as MBP g0s2, MBP-g0s2, g0s2-noHis_pMAL-c5E, or pHis6-MBP-g0s2. There is no His-tag in the expressed protein sequence.
Backbone vector is pMAL-c5E.
Construct design by James Zook.
Publication: Moran et al 2021 PLoS One 16, e0249164, https://doi.org/10.1371/journal.pone.0249164