Transfected plasmid DNA is incorporated into the nucleus via nuclear envelope reformation at telophase.
Haraguchi T, Koujin T, Shindo T, Bilir S, Osakada H, Nishimura K, Hirano Y, Asakawa H, Mori C, Kobayashi S, Okada Y, Chikashige Y, Fukagawa T, Shibata S, Hiraoka Y
Commun Biol. 2022 Jan 20;5(1):78. doi: 10.1038/s42003-022-03021-8. PubMed Article
Haraguchi T, Koujin T, Shindo T, Bilir S, Osakada H, Nishimura K, Hirano Y, Asakawa H, Mori C, Kobayashi S, Okada Y, Chikashige Y, Fukagawa T, Shibata S, Hiraoka Y
Commun Biol. 2022 Jan 20;5(1):78. doi: 10.1038/s42003-022-03021-8. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 179507 | pLacO-pEF1α-RFP | Expresses monomeric RFP in mammalian cells under a EF1α promoter |
| 179510 | pGFP-LacI | Expresses EGFP-LacI in mammalian cells |
| 179511 | hsLEMD2-pX330 | Encodes gRNA for human LEMD2 |
| 179524 | pAID1.2-EF1a-NmClover3-hsLem2 | Express OsTIR1 and mClover3-mAID-hsLem2 in mammalian cells under the control of EF1a promoter |