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pLacO-pEF1α-RFP
(Plasmid #179507)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 179507 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 179507-D50 50 μg of DNA in Tris buffer $495
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DNA 179507-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pEGFP-C1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4731
  • Total vector size (bp) 15000
  • Modifications to backbone
    replace CMV promoter for EF1α
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Growth instructions
    Recommend plate culture because liquid culture causes the reduction of the lacO repeat number.
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    monomeric RFP
  • Alt name
    mRFP
  • Species
    Discosoma sp. mushroom
  • Insert Size (bp)
    675
  • GenBank ID
  • Promoter EF1α

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site BglII (not destroyed)
  • 5′ sequencing primer -
  • 3′ sequencing primer -
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note, the exact number of Lac operators could not be determined via sequencing. Please refer to the diagnostic digest with AgeI-HF and SalI-HF confirming a ~7kb band representing approximately 200 copies of the lac operator within the plasmid.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLacO-pEF1α-RFP was a gift from Yasushi Hiraoka (Addgene plasmid # 179507 ; http://n2t.net/addgene:179507 ; RRID:Addgene_179507)

  • For your References section:

    Transfected plasmid DNA is incorporated into the nucleus via nuclear envelope reformation at telophase. Haraguchi T, Koujin T, Shindo T, Bilir S, Osakada H, Nishimura K, Hirano Y, Asakawa H, Mori C, Kobayashi S, Okada Y, Chikashige Y, Fukagawa T, Shibata S, Hiraoka Y. Commun Biol. 2022 Jan 20;5(1):78. doi: 10.1038/s42003-022-03021-8. 10.1038/s42003-022-03021-8 PubMed 35058555
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