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Synaptotagmin 7 is targeted to the axonal plasma membrane through gamma-secretase processing to promote synaptic vesicle docking in mouse hippocampal neurons.
Vevea JD, Kusick GF, Courtney KC, Chen E, Watanabe S, Chapman ER
Elife. 2021 Sep 20;10. pii: 67261. doi: 10.7554/eLife.67261.
PubMed Article

Plasmids from Article

ID Plasmid Purpose
179713pF(UG) hSyn SYT7alphaLentiviral expression of mouse Syt7
179714pF(UG) hSyn PP-HaloTag-SYT7alphaLentiviral expression of mouse Syt7 with N terminal HaloTag
179715pF(UG) hSyn SYT7alpha-HaloTagLentiviral expression of mouse Syt7 with C terminal HaloTag
179716pF(UG) hSyn SYT7alpha-HaloTag TMD Cys-AlaLentiviral expression of mouse Syt7 palmitoylation site mutant with C terminal HaloTag
179717pF(UG) hSyn SYT7alpha-HaloTag P2A PM-msGFPLentiviral expression of mouse Syt7 with C terminal HaloTag and PM-targeted msGFP
179718pF(UG) hSyn SYP-deltaTMD SYT7alphaLentiviral expression of synaptic vesicle-targeted mouse Syt7
179719pF(UG) hSyn SYP-deltaTMD SYT7alpha-HaloTagLentiviral expression of synaptic vesicle-targeted Halo tagged mouse Syt7
179720pF(UG) hSyn PM-SYT7alpha deltaTMDLentiviral expression of plasma membrane-targeted mouse Syt7
179721pF(UG) hSyn PM-SYT7alpha deltaTMD-HaloTagLentiviral expression of plasma membrane-targeted Halo tagged mouse Syt7
179722pF(UG) hSyn Lamp1-SYT7alpha deltaTMD Lentiviral expression of lysosome-targeted mouse Syt7
179723pF(UG) hSyn Lamp1-SYT7alpha deltaTMD-HaloTag Lentiviral expression of lysosome-targeted Halo tagged mouse Syt7
179724pF(UG) U6-SYT7 sgRNA 777 HaloTagU6-driven SYT7 gRNA and HaloTag lentiviral vector for CRISPR/HITI
179725pF(UG) hSyn spCas9human synapsin (hSyn)-driven spCas9 lentiviral vector for CRISPR/HITI
179726pF(UG) CamKII sf iGluSnFR S72ALentiviral expression of iGluSnFR biosensor for monitoring synaptic vesicle exocytosis and glutamate release
179727pF(UG) hSyn SYP-mRuby3Lentiviral expression of SYP-mRuby3 for use as a synaptic vesicle marker
179728pF(UG) hSyn LAMP1-msGFP (JV012)Lentiviral expression of LAMP1-msGFP for use as a lysosomal marker
179730pEF-mRuby3Mammalian expression of mRuby3 from the EF1 promoter

Antibodies from Article