Systematic functional analysis of SARS-CoV-2 proteins uncovers viral innate immune antagonists and remaining vulnerabilities.
Hayn M, Hirschenberger M, Koepke L, Nchioua R, Straub JH, Klute S, Hunszinger V, Zech F, Prelli Bozzo C, Aftab W, Christensen MH, Conzelmann C, Muller JA, Srinivasachar Badarinarayan S, Sturzel CM, Forne I, Stenger S, Conzelmann KK, Munch J, Schmidt FI, Sauter D, Imhof A, Kirchhoff F, Sparrer KMJ
Cell Rep. 2021 May 18;35(7):109126. PubMed Article
Hayn M, Hirschenberger M, Koepke L, Nchioua R, Straub JH, Klute S, Hunszinger V, Zech F, Prelli Bozzo C, Aftab W, Christensen MH, Conzelmann C, Muller JA, Srinivasachar Badarinarayan S, Sturzel CM, Forne I, Stenger S, Conzelmann KK, Munch J, Schmidt FI, Sauter D, Imhof A, Kirchhoff F, Sparrer KMJ
Cell Rep. 2021 May 18;35(7):109126. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 179961 | pCG_SARS-CoV1 NSP1 C-V5-tag | Mammalian expression vector for SARS-CoV-1 Nsp1, V5-tagged. Sequence codon-optimized. |
| 179962 | pCG_SARS-CoV1 NSP3 C-V5-tag | Mammalian expression vector for SARS-CoV-1 Nsp3, V5-tagged. Sequence codon-optimized. |
| 179963 | pCG_SARS-CoV1 NSP7 C-V5-tag | Mammalian expression vector for SARS-CoV-1 Nsp7, V5-tagged. Sequence codon-optimized. |
| 179964 | pCG_SARS-CoV1 NSP15 C-V5-tag | Mammalian expression vector for SARS-CoV-1 Nsp15, V5-tagged. Sequence codon-optimized. |
| 179965 | pCG_SARS-CoV1 NSP16 C-V5-tag | Mammalian expression vector for SARS-CoV-1 Nsp16, V5-tagged. Sequence codon-optimized. |
| 179966 | pCG_SARS-CoV1 E C-V5-tag | Mammalian expression vector for SARS-CoV-1 E, V5-tagged. Sequence codon-optimized. |
| 179967 | pCG_SARS-CoV1 M C-V5-tag | Mammalian expression vector for SARS-CoV-1 M, V5-tagged. Sequence codon-optimized. |
| 179968 | pCG_SARS-CoV1 N C-V5-tag | Mammalian expression vector for SARS-CoV-1 N, V5-tagged. Sequence codon-optimized. |
| 179969 | pCG_SARS-CoV1 C-V5-tag ORF3a | Mammalian expression vector for SARS-CoV-1 ORF3a, V5-tagged. Sequence codon-optimized. |
| 179970 | pCG_SARS-CoV1 ORF6 C-V5-tag | Mammalian expression vector for SARS-CoV-1 ORF6, V5-tagged. Sequence codon-optimized. |
| 179971 | pCG_SARS-CoV1 C-V5-tag ORF7a | Mammalian expression vector for SARS-CoV-1 ORF7a, V5-tagged. Sequence codon-optimized. |
| 179972 | pCG_SARS-CoV2 NSP1 C-V5-tag | Mammalian expression vector for SARS-CoV-2 Nsp1, V5-tagged. Sequence codon-optimized. |
| 179974 | pCG_SARS-CoV2 NSP7/bat-CoV RaTG13 C-V5-tag | Mammalian expression vector for SARS-CoV-2 Nsp7 or bat RaTG13 Nsp7, V5-tagged (SARS-CoV-2 Nsp7 and RaTG13 Nsp7 have the same amino acid sequence and codon optimized sequence). |
| 179975 | pCG_SARS-CoV2 NSP15 C-V5-tag | Mammalian expression vector for SARS-CoV-2 Nsp15, V5-tagged. Sequence codon-optimized. |
| 179976 | pCG_SARS-CoV2 NSP16 C-V5-tag | Mammalian expression vector for SARS-CoV-2 Nsp16, V5-tagged. Sequence codon-optimized. |
| 179977 | pCG_SARS-CoV2/bat-CoV RaTG13 E C-V5-tag | Mammalian expression vector for SARS-CoV-2 E or bat RaTG13 E, V5-tagged (SARS-CoV-2 E and RaTG13 E have the same amino acid sequence and codon optimized sequence). |
| 179978 | pCG_SARS-CoV2 M C-V5-tag | Mammalian expression vector for SARS-CoV-2 M, V5-tagged. Sequence codon-optimized. |
| 179979 | pCG_SARS-CoV2 N C-V5-tag | Mammalian expression vector for SARS-CoV-2 N, V5-tagged. Sequence codon-optimized. |
| 179980 | pCG_SARS-CoV2 ORF3a C-V5-tag | Mammalian expression vector for SARS-CoV-2 ORF3a, V5-tagged. Sequence codon-optimized. |
| 179981 | pCG_SARS-CoV2 ORF7a C-V5-tag | Mammalian expression vector for SARS-CoV-2 ORF7a, V5-tagged. Sequence codon-optimized. |
| 179982 | pCG_batCoV RaTG13 NSP1 C-V5-tag | Mammalian expression vector for RaTG13 Nsp1, V5-tagged. Sequence codon-optimized. |
| 179984 | pCG_batCoV RaTG13 NSP15 C-V5-tag | Mammalian expression vector for RaTG13 Nsp15, V5-tagged. Sequence codon-optimized. |
| 179985 | pCG_batCoV RaTG13 NSP16 C-V5-tag | Mammalian expression vector for RaTG13 Nsp16, V5-tagged. Sequence codon-optimized. |
| 179986 | pCG_batCoV RaTG13 M C-V5-tag | Mammalian expression vector for RaTG13 M, V5-tagged. Sequence codon-optimized. |
| 179987 | pCG_batCoV RaTG13 N C-V5-tag | Mammalian expression vector for RaTG13 N, V5-tagged. Sequence codon-optimized. |
| 179988 | pCG_batCoV RaTG13 ORF3a C-V5-tag | Mammalian expression vector for RaTG13 ORF3a, V5-tagged. Sequence codon-optimized. |
| 179989 | pCG_batCoV RaTG13 ORF7a C-V5-tag | Mammalian expression vector for RaTG13 ORF7a, V5-tagged. Sequence codon-optimized. |
| 179990 | pCG_SARS-CoV2 ORF8 C-V5-tag | Mammalian expression vector for SARS-CoV-2 ORF8, V5-tagged. Sequence codon-optimized. |