Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

Addgene
Reversible phase separation of ESCRT protein ALIX through tyrosine phosphorylation.
Elias RD, Zhu Y, Su Q, Ghirlando R, Zhang J, Deshmukh L
Sci Adv. 2023 Jul 14;9(28):eadg3913. doi: 10.1126/sciadv.adg3913. Epub 2023 Jul 14. PubMed Article

Plasmids from Article

ID Plasmid Purpose
180023GB1-PRD(800-868)-strepCodon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-ALIX(800-868)-Strep.
180024ALIX(P801G)-StrepCodon-optimized bacterial expression plasmid. Expresses ALIX(1-868, P801G)-Strep. P801G mutation facilitates expression in E. coli.
180025GB1-ALIX(P801G)Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-ALIX(1-868, P801G). P801G mutation facilitates expression in E. coli.
180026GB1-ΔN-CHMP4B(S184C)Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-CHMP4B(121-224, S184C).
180027GB1-ΔN-CHMP4C(S191C)Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-CHMP4C(121-233, S191C).
180029GB1-PRD(703-868, P801G)Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-ALIX(703-868, P801G). P801G mutation facilitates expression in E. coli.
186793GB1-CEP55(160-217, S215C)Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-CEP55(160-217, S215C)
190783GB1-ΔN-CHMP4C(G154C)Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-CHMP4C(121-233, G154C)
190784GB1-ΔN-CHMP4C(M165C)Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-CHMP4C(121-233, M165C)
191191ALIX-mNeonGreenFull-length ALIX with C-terminal mNeonGreen tag.
191192ALIX(P801G)-mNeonGreenFull-length ALIX with P801G mutation in proline-rich domain; C-terminally tagged with mNeonGreen.
191193ALIX(1-702)-mNeonGreenTruncated form of ALIX that lacks C-terminal proline-rich domain; C-terminally tagged with mNeonGreen.
199242GB1-ΔN-CHMP4C(S191C,I225A,L228A,W231A)Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-CHMP4C(121-233, S191C,I225A,L228A,W231A)

Antibodies from Article