Reversible phase separation of ESCRT protein ALIX through tyrosine phosphorylation.
Elias RD, Zhu Y, Su Q, Ghirlando R, Zhang J, Deshmukh L
Sci Adv. 2023 Jul 14;9(28):eadg3913. doi: 10.1126/sciadv.adg3913. Epub 2023 Jul 14.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 180023 | GB1-PRD(800-868)-strep | Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-ALIX(800-868)-Strep. |
| 180024 | ALIX(P801G)-Strep | Codon-optimized bacterial expression plasmid. Expresses ALIX(1-868, P801G)-Strep. P801G mutation facilitates expression in E. coli. |
| 180025 | GB1-ALIX(P801G) | Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-ALIX(1-868, P801G). P801G mutation facilitates expression in E. coli. |
| 180026 | GB1-ΔN-CHMP4B(S184C) | Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-CHMP4B(121-224, S184C). |
| 180027 | GB1-ΔN-CHMP4C(S191C) | Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-CHMP4C(121-233, S191C). |
| 180029 | GB1-PRD(703-868, P801G) | Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-ALIX(703-868, P801G). P801G mutation facilitates expression in E. coli. |
| 186793 | GB1-CEP55(160-217, S215C) | Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-CEP55(160-217, S215C) |
| 190783 | GB1-ΔN-CHMP4C(G154C) | Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-CHMP4C(121-233, G154C) |
| 190784 | GB1-ΔN-CHMP4C(M165C) | Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-CHMP4C(121-233, M165C) |
| 191191 | ALIX-mNeonGreen | Full-length ALIX with C-terminal mNeonGreen tag. |
| 191192 | ALIX(P801G)-mNeonGreen | Full-length ALIX with P801G mutation in proline-rich domain; C-terminally tagged with mNeonGreen. |
| 191193 | ALIX(1-702)-mNeonGreen | Truncated form of ALIX that lacks C-terminal proline-rich domain; C-terminally tagged with mNeonGreen. |
| 199242 | GB1-ΔN-CHMP4C(S191C,I225A,L228A,W231A) | Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-CHMP4C(121-233, S191C,I225A,L228A,W231A) |