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(Plasmid #191191)


This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 191191 Standard format: Plasmid sent in bacteria as agar stab 1 $85


  • Vector backbone
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 5396
  • Total vector size (bp) 8723
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
  • Alt name
  • Species
    H. sapiens (human), Synthetic
  • Insert Size (bp)
  • Entrez Gene
    PDCD6IP (a.k.a. AIP1, ALIX, DRIP4, HP95, MCPH29)
  • Promoter CMV
  • Tags / Fusion Proteins
    • 6xHIS (N terminal on insert)
    • mNeonGreen (C terminal on insert)

Cloning Information

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    ALIX cDNA was provided by Lalit Deshmukh (UCSD)

Terms and Licenses

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    ALIX-mNeonGreen was a gift from Jin Zhang (Addgene plasmid # 191191 ; ; RRID:Addgene_191191)
  • For your References section:

    Reversible phase separation of ESCRT protein ALIX through tyrosine phosphorylation. Elias RD, Zhu Y, Su Q, Ghirlando R, Zhang J, Deshmukh L. Sci Adv. 2023 Jul 14;9(28):eadg3913. doi: 10.1126/sciadv.adg3913. Epub 2023 Jul 14. 10.1126/sciadv.adg3913 PubMed 37450591