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A user-friendly and streamlined protocol for CRISPR/Cas9 genome editing in budding yeast.
Novarina D, Koutsoumpa A, Milias-Argeitis A
STAR Protoc. 2022 Jun 10;3(2):101358. doi: 10.1016/j.xpro.2022.101358. eCollection 2022 Jun 17.
PubMed Article

Plasmids from Article

ID Plasmid Purpose
180282pYTK-DN1Cas9 cassette (TU1)
180283pYTK-DN2Spacer cassette (TU3)
180284pYTK-DN3Spacer cassette (TU2)
180285pYTK-DN4KanR multi-gene yeast expression vector (GFP-dropout)
180286pYTK-DN5NatR multi-gene yeast expression vector (GFP-dropout)
180287pYTK-DN6HygR multi-gene yeast expression vector (GFP-dropout)

Antibodies from Article