Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

Addgene
Disrupted expression of mitochondrial NCLX sensitizes neuroglial networks to excitotoxic stimuli and renders synaptic activity toxic.
Hagenston AM, Yan J, Bas-Orth C, Tan Y, Sekler I, Bading H
J Biol Chem. 2021 Dec 20:101508. doi: 10.1016/j.jbc.2021.101508.
PubMed Article

Plasmids from Article

ID Plasmid Purpose
181870pAAV-shNCLX-1Expresses NCLX-targeted shRNA under control of the U6 promoter and mCherry under control of the CaMKIIa promoter
181871pAAV-shNCLX-2Expresses NCLX-targeted shRNA under control of the U6 promoter and mCherry under control of the CaMKIIa promoter
181872pAAV-EGFP.T2A.NCLX.3'UTRExpresses EGFP and murine NCLX (separated by a T2A cleavage site) under control of the synthetic CAG promoter (includes a large portion of the Slc8b1 3'UTR)
181873pAAV-EGFP.T2A.NCLXExpresses EGFP and murine NCLX (separated by a T2A cleavage site) under control of the synthetic CAG promoter
181874pAAV-4mtD3cpvExpresses the FRET-based mitochondrial calcium indicator 4mtD3cpv under control of the CaMKIIa promoter
181875pAAV-shCTRLExpresses a control shRNA with no known targets in the mouse genome under control of the U6 promoter and mCherry under control of the CaMKIIa promoter

Antibodies from Article