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Real-time detection of response regulator phosphorylation dynamics in live bacteria.
Butcher RJ, Tabor JJ
Proc Natl Acad Sci U S A. 2022 Aug 30;119(35):e2201204119. doi: 10.1073/pnas.2201204119. Epub 2022 Aug 22. PubMed Article

Plasmids from Article

ID Plasmid Purpose
181944pRB035aTc-inducible expression of mNeonGreen
181945pRB121aTc-inducible expression of dimerized mNeonGreen (mNG-mNG)
181946pRB116.2aTc-inducible expression of TorR with C-terminal mNeonGreen fusion. Also contains mCherry under TorR-controlled promoter PtorCAD129
181947pRB120.2aTc-inducible expression of TorR with N-terminal mNeonGreen fusion. Also contains mCherry under TorR-controlled promoter PtorCAD129
181948pRB113.2aTc-inducible expression of NarL with C-terminal mNeonGreen fusion. Also contains mCherry under NarL-controlled promoter PdcuS
181949pRB117.2aTc-inducible expression of NarL with N-terminal mNeonGreen fusion. Also contains mCherry under NarL-controlled promoter PdcuS
181950pRB133.2aTc-inducible expression of PhoP with C-terminal mNeonGreen fusion. Also contains mCherry under PhoP-controlled promoter PvirK
181951pKD317.8IPTG-inducible expression of NarX
181952pKB083IPTG-inducible expression of PhoQ

Antibodies from Article