pRB121
(Plasmid #181945)

• Purpose: aTc-inducible expression of dimerized mNeonGreen (mNG-mNG)
• Depositing Lab: Jeffrey Tabor
• Publication: Butcher et al Proc Natl Acad Sci U S A. 2022 Aug 30;119(35):e2201204119. doi: 10.1073/pnas.2201204119. Epub 2022 Aug 22.

Backbone

• Vector backbone: ColE1
• Backbone size w/o insert (bp): 1706
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol, 25 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: mNeonGreen-mNeonGreen; tetR
• Alt name: mNG-mNG
• Species: Synthetic
• Mutation: See Depositor Comments Below
• GenBank ID: mNG - QIK01997; tetR - WP_000470728
• Entrez Gene: AX04_RS23485 (a.k.a. AX04_RS23485, AX04_23495)
• Promoter: mNG-mNG-PLtetO-1; tetR-J23106

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BsaI (destroyed during cloning)
• 3′ cloning site: BsaI (destroyed during cloning)

Resource Information

• Supplemental Documents:
  • prb121.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • mNeonGreen Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note: Plasmid contains E7G and E253G mutations in the mNeonGreen-mNeonGreen insert. These mutations are in the unstructured N-terminal region prior to the classic fluorescent protein beta-barrel shape and are not known to affect plasmid function.