In situ probe and inhibitory RNA synthesis using streamlined gene cloning with Gibson assembly.
Wolff A, Wagner C, Wolf J, Lobo D
STAR Protoc. 2022 Jun 14;3(3):101458. doi: 10.1016/j.xpro.2022.101458. eCollection 2022 Sep 16. PubMed Article
Wolff A, Wagner C, Wolf J, Lobo D
STAR Protoc. 2022 Jun 14;3(3):101458. doi: 10.1016/j.xpro.2022.101458. eCollection 2022 Sep 16. PubMed Article
Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
182263 | pDL1 | Gibson cloning vector for synthesis of single and double stranded RNA |
182264 | pAW2-Smed-Slit-1 | dsRNA and riboprobe synthesis for Schmidtea mediterranea Slit-1 |