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mtIF3 is locally translated in axons and regulates mitochondrial translation for axonal growth.
Lee S, Park D, Lim C, Kim JI, Min KT
BMC Biol. 2022 Jan 7;20(1):12. doi: 10.1186/s12915-021-01215-w.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
182367pAAV2-mtIF3 shRNA_TagRFP657shRNA targeting mtIF3 mRNA
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182368pDendra2-5'UTR(GAPDH)-Dendra2-3'UTR(GAPDH)Dendra2-based translation reporter
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182369pcDNA6-5'UTR(mtIF3)-Dendra2-3'UTR(mtIF3)Dendra2-based translation reporter
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182370pcDNA6-5'UTR(mtIF3)-mtIF3-Dendra2-3'UTR(mtIF3)Dendra2-based translation reporter
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182371pcDNA6-mtIF3-Dendra2Dendra2-based translation reporter
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182373pcDNA6-MRPL50-VC155mVenus-based mito-riboBiFC vector (Negative control)
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182374pcDNA6-MRPS6-VN172mVenus-based mito-riboBiFC vector
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182375pcDNA6-MRPS16-VN172mVenus-based mito-riboBiFC vector (Negative control)
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182376psiCheck2-SV40p-3xFLAG-CMVp-mito-mTFP1Dual expression construct encoding 3xFLAG and mito-mTFP1 from separate promoters
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182377psiCheck2-SV40p-3xFLAG-HSVTKp-mCherryDual expression construct encoding 3xFLAG and mCherry from separate promoters
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182378psiCheck2-SV40p-5'UTR-mtIF3(SR)-3xFLAG-3'UTR-CMVp-mito-mTFP1Dual expression construct encoding shRNA-resistant mtIF3 and mito-mTFP1 from separate promoters
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182379psiCheck2-SV40p-5'UTR-mtIF3(SR)-3xFLAG-3'UTR-HSVTKp-mCherryDual expression construct encoding shRNA-resistant mtIF3 and mCherry from separate promoters
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182380psiCheck2-SV40p-mtIF3(SR)-3xFLAG-CMVp-mito-mTFP1Dual expression construct encoding shRNA-resistant mtIF3 and mito-mTFP1 from separate promoters
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182381psiCheck2-SV40p-mtIF3(SR)-3xFLAG-HSVTKp-mCherryDual expression construct encoding shRNA-resistant mtIF3 and mCherry from separate promoters
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Antibodies from Article