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Direct Observation of Compartment-Specific Localization and Dynamics of Voltage-Gated Sodium Channels.
Liu H, Wang HG, Pitt GS, Liu ZJ
J Neurosci. 2022 Jun 6;42(28):5482-98. doi: 10.1523/JNEUROSCI.0086-22.2022. PubMed Article

Plasmids from Article

ID Plasmid Purpose
182554piggybac-Syn-mScn8a-3xV5Expresses Nav1.6 tagged with 3xV5
182555piggybac-Syn-mScn8a-3xHAExpresses Nav1.6 tagged with 3xHA
182560piggybac-Syn-mScn8a_ICL1-sfGFP-P2A-mCherryExpresses Nav1.6 ICL1 tagged with sfGFP-P2A-mCherry
182561PX552-mScn2a-3xV5-EF1-smFP-flagFor mouse Nav1.2 knockin with 3xV5 at the C-terminal
182562PX552-mScn8a-3xV5-EF1-smFP-flagFor mouse Nav1.6 knockin with 3xV5 at the C-terminal
182563PX552-mScn8a-3xHA-Syn-smFP-flagFor mouse Nav1.6 knockin with 3xHA at the C-terminal
182564PX552-mScn2a-HaloTag-V5-EF1-sfGFPFor mouse Nav1.2 knockin with HaloTag-V5 at the C-terminal
182565PX552-mScn8a-HaloTag-V5-EF1-sfGFPFor mouse Nav1.6 knockin with HaloTag-V5 at the C-terminal
182566PX552-rScn2a-3xV5-Syn-miRFP670For rat Nav1.2 knockin with 3xV5 at the extracellular loop

Antibodies from Article