Increased prime edit rates in KCNQ2 and SCN1A via single nicking all-in-one plasmids.
Dirkx N, Weuring WJ, De Vriendt E, Smal N, van de Vondervoort J, van 't Slot R, Koetsier M, Zonnekein N, De Pooter T, Weckhuysen S, Koeleman BPC
BMC Biol. 2023 Jul 13;21(1):156. doi: 10.1186/s12915-023-01646-7. PubMed Article
Dirkx N, Weuring WJ, De Vriendt E, Smal N, van de Vondervoort J, van 't Slot R, Koetsier M, Zonnekein N, De Pooter T, Weckhuysen S, Koeleman BPC
BMC Biol. 2023 Jul 13;21(1):156. doi: 10.1186/s12915-023-01646-7. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 184444 | pLenti_Ef1a:PE2-P2A-hMLH1dn | Single EF1a-driven PE2-P2A-hMLH1dn (Addgene #174828) and PaqCI restriction sites for pegRNA cloning. Lentiviral backbone and AmpR. |
| 184445 | pLenti_Ef1a:PE2-P2A-GFP | Single EF1a-driven PE2-P2A-GFP (Addgene #132776) and PaqCI restriction sites for pegRNA cloning. Lentiviral backbone and AmpR. |