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A Markerless CRISPR-Mediated System for Genome Editing in Candida auris Reveals a Conserved Role for Cas5 in the Caspofungin Response.
Ennis CL, Hernday AD, Nobile CJ
Microbiol Spectr. 2021 Dec 22;9(3):e0182021. doi: 10.1128/Spectrum.01820-21. Epub 2021 Nov 3. PubMed Article

Plasmids from Article

ID Plasmid Purpose
174405pCE27C. auris LEUpOUT NAT marker gRNA expression construct. Use with pCE35 CAS9 expression construct.
174409pCE35C. auris LEUpOUT NAT marker CAS9 expression construct. Use with pCE27 gRNA expression construct.
174432pCE38C. auris LEUpOUT HYG marker CAS9 expression construct. Use with pCE41 gRNA expression construct.
174433pCE41C. auris LEUpOUT HYG marker gRNA expression construct. Use with pCE38 CAS9 expression construct.
174434pCE1Candida optimized monomeric eGFP with RIPLING linker sequence.

Antibodies from Article