Recruitment of clathrin to intracellular membranes is sufficient for vesicle formation.
Kuey C, Sittewelle M, Larocque G, Hernandez-Gonzalez M, Royle SJ
Elife. 2022 Jul 19;11. pii: 78929. doi: 10.7554/eLife.78929. PubMed Article
Kuey C, Sittewelle M, Larocque G, Hernandez-Gonzalez M, Royle SJ
Elife. 2022 Jul 19;11. pii: 78929. doi: 10.7554/eLife.78929. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 186573 | pMito-dCherry-FRB | Expression of "dark MitoTrap" - an FRB-domain targeted to the mitochondria that is the same size as pMito-mCherry-FRB but is non-fluorescent - in human cells |
| 186574 | FRB-mCherry-Sec61β | Expression of FRB-mCherry-tagged ER protein Sec61beta in mammalian cells |
| 186575 | FRB-mCherry-Giantin | Expression of FRB-mCherry on Golgi membrane with Giantin transmembrane (residues 3131-3259) |
| 186576 | Lamp1-mCherry-FRB | Expression of mCherry-FRB at the outer surface of the lysosome using LAMP1 |
| 186577 | FKBP-AP180-GFP | Expression of the fusion protein FKBP-AP180-GFP. Uses rat AP180 residues 328-896. |
| 186578 | σ1-mCherry | Expression of adaptor protein 1 (AP1) sigma subunit AP1S1 tagged with mCherry |
| 186579 | σ2-mCherry | Expression of adaptor protein 2 (AP2) sigma subunit AP2S1 tagged with mCherry |
| 186580 | σ3-mCherry | Expression of adaptor protein 3 (AP3) sigma subunit AP3S1 tagged with mCherry |