FKBP-AP180-GFP
(Plasmid #186577)

• Purpose: Expression of the fusion protein FKBP-AP180-GFP. Uses rat AP180 residues 328-896.
• Depositing Lab: Stephen Royle
• Publication: Kuey et al Elife. 2022 Jul 19;11. pii: 78929. doi: 10.7554/eLife.78929.

Backbone

• Vector backbone: pEGFP-N1
• Backbone manufacturer: Clontech
• Total vector size (bp): 7369
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: AP180
• Alt name: SNAP91
• Species: R. norvegicus (rat)
• Entrez Gene: Snap91 (a.k.a. Ap180)
• Tag / Fusion Protein:
  • FKBP N-terminal GFP C-terminal

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: AgeI (not destroyed)
• 5′ sequencing primer: pCMV

Resource Information

• Supplemental Documents:
  • pFKBP-AP180C-EGFP.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note: The plasmid is difficult to sequence near bases 2140-2220 that are within the insert.