Functional editing of endogenous genes through rapid selection of cell pools (Rapid generation of endogenously tagged genes in Drosophila ovarian somatic sheath cells).
Meng Q, Stoyko D, Andrews CM, Konstantinidou P, Genzor P, O T, Elchert AR, Benner L, Sobti S, Katz EY, Haase AD
Nucleic Acids Res. 2022 May 27. pii: 6594084. doi: 10.1093/nar/gkac448. PubMed Article
Meng Q, Stoyko D, Andrews CM, Konstantinidou P, Genzor P, O T, Elchert AR, Benner L, Sobti S, Katz EY, Haase AD
Nucleic Acids Res. 2022 May 27. pii: 6594084. doi: 10.1093/nar/gkac448. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 186650 | puc19_N_tag | Empty donor plasmid. |
| 186651 | Donor_N_FH_Piwi | Donor plasmid to endogenously tag piwi gene in Drosophila melanogaster ovarian somatic sheet (OSS) cells. |
| 186652 | Cas9_nonPuro_OSS | Cas9 plasmid to express the Cas9 in OSS cells |
| 186653 | piwi_sgRNA | piwi sgRNA plasmid |
| 186655 | Donor_N_FH_Shu | Donor plasmid to endogenously tag Shu gene in Drosophila melanogaster ovarian somatic sheet (OSS) cells. |
| 186656 | Shu_sgRNA | shu sgRNA plasmid |
| 186657 | pDonor_N_FH_GLE | Donor plasmid to endogenously tag GLE gene in Drosophila melanogaster ovarian somatic sheet (OSS) cells. |
| 186658 | GLE_sgRNA | GLE sgRNA plasmid |
| 186659 | PUC19_C_tag | Empty donor plasmid for C-terminal tag of 3xFlag-3xHA. |
| 186660 | Donor_C_FH_VIR | Donor plasmid to endogenously tag VIR gene in Drosophila melanogaster ovarian somatic sheet (OSS) cells. |
| 186661 | VIR_sgRNA | VIR sgRNA plasmid |
| 186662 | Donor_C_FH_Nbr | Donor plasmid to endogenously tag Nbr gene in Drosophila melanogaster at the C-terminal. |
| 186663 | Nbr_C_sgRNA1 | Nbr C-tag sgRNA1 plasmid |
| 186664 | Nbr_C_sgRNA2 | Nbr C-tag sgRNA2 plasmid |
| 186667 | Donor_ANKRD1_KO_Puro | Donor plasmid to endogenously knock out the ANKRD1 in Hek293 cells. eEF1a promoter, puromycin and polyA sequence is inserted between two homology arms. |
| 186668 | Donor_ANKRD1_KO_Hygro | Donor plasmid to endogenously knock out the ANKRD1 in Hek293 cells. eEF1a promoter, hygromycin and polyA sequence is inserted between two homology arms. |
| 186669 | Cas9_ANKRD1_sgRNA2 | Plasmid containing Cas9 and ANKRD1 sgRNA2 , sgRNA sequence: cggtcagcttatatagct. |
| 186670 | Donor_YTHDF2_KO_Puro | Donor plasmid to endogenously knock out the YTHDF2 in Hek293 cells. Puromycin and polyA sequence is inserted between two homology arms. |
| 186671 | Donor_YTHDF2_KO_Hygro | Donor plasmid to endogenously knock out the YTHDF2 in Hek293 cells. Hygromycin and polyA sequence is inserted between two homology arms. |
| 186672 | Donor_YTHDF2_KO_Neo | Donor plasmid to endogenously knock out the ANKRD1 in Hek293 cells. Neomycin and polyA sequence is inserted between two homology arms. |
| 186673 | Cas9_YTHDF2_sgRNA | YTHDF2 sgRNA plasmid |
| 186674 | pUC19_Ntag_BbsI-Mammalian | N-terminal tag cassette with BbsI restriction sites for CRISPR donor plasmid. |