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Ingestion of single guide RNAs induces gene overexpression and extends lifespan in C. elegans via CRISPR activation.
Fischer F, Benner C, Goyala A, Grigolon G, Vitiello D, Wu J, Zarse K, Ewald CY, Ristow M
J Biol Chem. 2022 May 27:102085. doi: 10.1016/j.jbc.2022.102085.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
177783L4440_BioBrick-sgRNAOverexpression of sgRNAs in E. coli HT115, sgRNA overexpression scaffold; for ingestion by C. elegans
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177784L4440_BioBrick-hif-1-sgRNA-AOverexpression of sgRNAs in E. coli HT115 (targeting C. elegans hif-1 promoter)
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177785L4440_BioBrick-hif-1-sgRNA-BOverexpression of sgRNAs in E. coli HT115 (targeting C. elegans hif-1 promoter)
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177786L4440_BioBrick-hsf-1-sgRNA-AOverexpression of sgRNAs in E. coli HT115 (targeting C. elegans hsf-1 promoter)
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177787L4440_BioBrick-hsf-1-sgRNA-BOverexpression of sgRNAs in E. coli HT115 (targeting C. elegans hsf-1 promoter)
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177788pdestMB14_sur5p-dCas9-VP64Overexpression of dCas9-VP64 in C. elegans
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177811L4440_BioBrick-SCR-sgRNA-AOverexpression of sgRNAs in E. coli HT115 (scrambled targeting sequences)
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177812L4440_BioBrick-SCR-sgRNA-BOverexpression of sgRNAs in E. coli HT115 (scrambled targeting sequences)
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Antibodies from Article