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Selection of a histidine auxotrophic Marchantia polymorpha strain with an auxotrophic selective marker.
Fukushima T, Kodama Y
Plant Biotechnol (Tokyo). 2022 Dec 25;39(4):345-354. doi: 10.5511/plantbiotechnology.22.0810a. Epub 2022 Nov 26.
PubMed Article

Plasmids from Article

ID Plasmid Purpose
188551pMpGE_En03-gR082Gateway entry vector for sgRNA targeted to MpIGPD.Transient expression of sgRNA targeted to MpIGPD in Marchantia polymorpha.
188552pMpGE_En03-gR085Gateway entry vector for sgRNA targeted to MpIGPD.Transient expression of sgRNA targeted to MpIGPD in Marchantia polymorpha.
188553pMpGE010-gR082Binary vector for CRISPR/Cas9 targeted to MpIGPD in Marchantia polymorpha (for Agrobacterium-mediated genetic transformation)
188554pMpGE010-gR085Binary vector for CRISPR/Cas9 targeted to MpIGPD in Marchantia polymorpha (for Agrobacterium-mediated genetic transformation)
188555pMpGWB303-MpIGPDmBinary vector for MpIGPDm and mALS as selective markers in Marchantia polymorpha (igpd mutants)
188556pMpGWBhis03Binary vector with MpIGPDm as a selective marker in Marchantia polymorpha (igpd mutants)
188557pMpGWBhis03-CitrineBinary vector for the expression of Citrine with MpIGPDm selective marker in Marchantia polymorpha (igpd mutants) .
188558pMpGWBhis03-Citrine-NLSBinary vector for the expression of Citrine-NLS with MpIGPDm selective marker in Marchantia polymorpha (igpd mutants) .

Antibodies from Article