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Dissecting protein tyrosine phosphatase signaling by engineered chemogenetic control of its activity.
Fauser J, Huyot V, Matsche J, Szynal BN, Alexeev Y, Kota P, Karginov AV
J Cell Biol. 2022 Aug 1;221(8). pii: 213352. doi: 10.1083/jcb.202111066. Epub 2022 Jul 13. PubMed Article

Plasmids from Article

ID Plasmid Purpose
188655RapR-Shp2-mVenus-flagRapamycin regulated Shp2 phosphatase with mVenus and flag tag
188656flag-RapR-PTP1BRapamycin regulated PTP1B with flag tag
188657mVenus-RapR-PTPPestRapamycin regulated PTPPest with mVenus tag
188658FRB-GFP-Gab1(Y628F/Y660F)FRB coupled to GFP tagged Gab1 with Shp2 binding sites mutated to Phe
188659FRB-GFP-Gab2(Y604F/Y634F)FRB coupled to GFP tagged Gab2 with Shp2 binding sites mutated to Phe
188660mVenus-FAK(397)FRBmVenus tagged Focal Adhesion Kinase with FRB inserted at Y397

Antibodies from Article