A set of Saccharomyces cerevisiae integration vectors for fluorescent dye labeling of proteins.
Baek I, Le SN, Jeon J, Chun Y, Reed C, Buratowski S
G3 (Bethesda). 2022 Aug 9. pii: 6659097. doi: 10.1093/g3journal/jkac201. PubMed Article
Baek I, Le SN, Jeon J, Chun Y, Reed C, Buratowski S
G3 (Bethesda). 2022 Aug 9. pii: 6659097. doi: 10.1093/g3journal/jkac201. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 188914 | pBS-SKII-3XHA-fSNAP-Hygromycin | Integration cassette to create C-terminal fusions for fluorescent tagging |
| 188915 | pBS-SKII-3XHA-fSNAP-Phleomycin | Integration cassette to create C-terminal fusions for fluorescent tagging |
| 188916 | pBS-SKII-3XHA-fSNAP-NAT | Integration cassette to create C-terminal fusions for fluorescent tagging |
| 188917 | pBS-SKII-3XHA-fSNAP-URA | Integration cassette to create C-terminal fusions for fluorescent tagging |
| 188918 | pBS-SKII-3XHA-fSNAP-Kan | Integration cassette to create C-terminal fusions for fluorescent tagging |
| 188919 | pBS-SKII-3XHA-fCLIP-Hygromycin | Integration cassette to create C-terminal fusions for fluorescent tagging |
| 188920 | pBS-SKII-3XHA-fCLIP-Phleomycin | Integration cassette to create C-terminal fusions for fluorescent tagging |
| 188921 | pBS-SKII-3XHA-fCLIP-NAT | Integration cassette to create C-terminal fusions for fluorescent tagging |
| 188922 | pBS-SKII-3XHA-fCLIP-URA | Integration cassette to create C-terminal fusions for fluorescent tagging |
| 188923 | pBS-SKII-3XHA-fCLIP-Kan | Integration cassette to create C-terminal fusions for fluorescent tagging |
| 188924 | pBS-SKII-3XHA-eDHFR-Hygromycin | Integration cassette to create C-terminal fusions for fluorescent tagging |
| 188925 | pBS-SKII-3XHA-eDHFR-Phleomycin | Integration cassette to create C-terminal fusions for fluorescent tagging |
| 188926 | pBS-SKII-3XHA-eDHFR-NAT | Integration cassette to create C-terminal fusions for fluorescent tagging |
| 188927 | pBS-SKII-3XHA-eDHFR-URA | Integration cassette to create C-terminal fusions for fluorescent tagging |
| 188928 | pBS-SKII-3XHA-eDHFR-Kan | Integration cassette to create C-terminal fusions for fluorescent tagging |
| 188929 | pBS-SKII-3XHA-HALO-Hygromycin | Integration cassette to create C-terminal fusions for fluorescent tagging |
| 188930 | pBS-SKII-3XHA-HALO-Phleomycin | Integration cassette to create C-terminal fusions for fluorescent tagging |
| 188931 | pBS-SKII-3XHA-HALO-NAT | Integration cassette to create C-terminal fusions for fluorescent tagging |
| 188932 | pBS-SKII-3XHA-HALO-URA | Integration cassette to create C-terminal fusions for fluorescent tagging |
| 188933 | pBS-SKII-3XHA-HALO-Kanamycin | Integration cassette to create C-terminal fusions for fluorescent tagging |
| 188934 | pBS-SKII-URA-GAL1-DHFR-3xHA | Integration cassette to create N-terminal fusions for fluorescent tagging |
| 188935 | pBS-SKII-URA-GAL1-fSNAP-3xHA | Integration cassette to create N-terminal fusions for fluorescent tagging |
| 188936 | pBS-SKII-URA-GAL1-fCLIP-3xHA | Integration cassette to create N-terminal fusions for fluorescent tagging |
| 188937 | pBS-SKII-URA-GAL1-HALO-3xHA | Integration cassette to create N-terminal fusions for fluorescent tagging |
| 188938 | pBS-SKII-URA-GAL1-fSNAP-GS | Integration cassette to create N-terminal fusions for fluorescent tagging |
| 188939 | pBS-SKII-URA-GAL1-fCLIP-GS | Integration cassette to create N-terminal fusions for fluorescent tagging |
| 188940 | pBS-SKII-URA-GAL1-HALO-GS | Integration cassette to create N-terminal fusions for fluorescent tagging |
| 188941 | pET28-his6-HA3-SNAPf | T7 expression vector for making his6- and HA3-tagged SNAP protein |
| 188942 | pET28-his6-HA3-CLIP | T7 expression vector for making his6- and HA3-tagged CLIP protein |
| 188943 | pET28-his6-HA3-HALO | T7 expression vector for making his6- and HA3-tagged HALO protein |