pBS-SKII-3XHA-fSNAP-Hygromycin
(Plasmid
#188914)
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PurposeIntegration cassette to create C-terminal fusions for fluorescent tagging
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 188914 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepBlueScript II SK (+)
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Vector typeYeast Expression
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert name3XHA-fSNAP-Hygromycin
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SpeciesSynthetic
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBS-SKII-3XHA-fSNAP-Hygromycin was a gift from Stephen Buratowski (Addgene plasmid # 188914 ; http://n2t.net/addgene:188914 ; RRID:Addgene_188914) -
For your References section:
A set of Saccharomyces cerevisiae integration vectors for fluorescent dye labeling of proteins. Baek I, Le SN, Jeon J, Chun Y, Reed C, Buratowski S. G3 (Bethesda). 2022 Aug 9. pii: 6659097. doi: 10.1093/g3journal/jkac201. 10.1093/g3journal/jkac201 PubMed 35944214
