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pDZ274 (pLEU MET25pro MCP-2x-yeGFP)
(Plasmid #45929)


Item Catalog # Description Quantity Price (USD)
Plasmid 45929 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Vector type
    Yeast Expression
  • Selectable markers

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
  • Alt name
    MS2 coat protein
  • Insert Size (bp)
  • Promoter MET25
  • Tag / Fusion Protein
    • 2x-yeGFP (C terminal on insert)

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer AATGGCACGTGAAGCTGTCG
  • 3′ sequencing primer GGCCGCAAATTAAAGCC
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDZ274 (pLEU MET25pro MCP-2x-yeGFP) was a gift from Robert Singer & Daniel Zenklusen (Addgene plasmid # 45929 ; ; RRID:Addgene_45929)
  • For your References section:

    Single-molecule analysis of gene expression using two-color RNA labeling in live yeast. Hocine S, Raymond P, Zenklusen D, Chao JA, Singer RH. Nat Methods. 2013 Feb;10(2):119-21. doi: 10.1038/nmeth.2305. Epub 2012 Dec 23. 10.1038/nmeth.2305 PubMed 23263691