Addgene: Engineered serum markers for non-invasive monitoring of gene expression in the brain.

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Engineered serum markers for non-invasive monitoring of gene expression in the brain.
Lee S, Nouraein S, Kwon JJ, Huang Z, Wojick JA, Xia B, Corder G, Szablowski JO
Nat Biotechnol. 2024 Jan 10. doi: 10.1038/s41587-023-02087-x. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
189624	pAAV-hSyn-Cluc-RMA	Expresses Cluc-RMA under the hSyn promoter. Cluc-RMA for monitoring neuronal transduction.
189627	pAAV-hSyn-hM3D(Gq)::RAM-d2tTA	Expresses hM3D(Gq) under the hSyn promoter, and d2tTA under the pRAM (synthetic Fos) promoter. Plasmid 1 for recording Fos expression.
189628	pAAV-TRE-Gluc-RMA-IRES-EGFP	Expresses Gluc-RMA and EGFP driven by the Tet Response Element (TRE). Plasmid 2 for recording Fos expression.
189629	pAAV-hSyn-Gluc-RMA-IRES-EGFP	Expresses Gluc-RMA and EGFP under the hSyn promoter. Gluc-RMA for monitoring neuronal transduction.
189630	pAAV-hSyn-DIO-Gluc-RMA-IRES-EGFP	Expresses Gluc-RMA and EGFP in the presence of Cre recombinase. Double-floxed Gluc-RMA and EGFP for monitoring Cre-expressing neuronal populations.

Antibodies from Article