Engineering of NEMO as calcium indicators with large dynamics and high sensitivity.
Li J, Shang Z, Chen JH, Gu W, Yao L, Yang X, Sun X, Wang L, Wang T, Liu S, Li J, Hou T, Xing D, Gill DL, Li J, Wang SQ, Hou L, Zhou Y, Tang AH, Zhang X, Wang Y
Nat Methods. 2023 Jun;20(6):918-924. doi: 10.1038/s41592-023-01852-9. PubMed Article
Li J, Shang Z, Chen JH, Gu W, Yao L, Yang X, Sun X, Wang L, Wang T, Liu S, Li J, Hou T, Xing D, Gill DL, Li J, Wang SQ, Hou L, Zhou Y, Tang AH, Zhang X, Wang Y
Nat Methods. 2023 Jun;20(6):918-924. doi: 10.1038/s41592-023-01852-9. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 189930 | pCDNA3.1(+)-NEMOm | Mammalian expression of calcium sensor with ultra-high dynamics and sensitivity |
| 189931 | pCDNA3.1(+)-NEMOs | Mammalian expression of calcium sensor with ultra-high dynamics and sensitivity |
| 189932 | pCDNA3.1(+)-NEMOf | Mammalian expression of calcium sensor with ultra-high dynamics and sensitivity |
| 189933 | pCDNA3.1(+)-NEMOc | Mammalian expression of calcium sensor with ultra-high dynamics and sensitivity |
| 189934 | pCDNA3.1(+)-NEMOb | Mammalian expression of calcium sensor with ultra-high dynamics and sensitivity |