Circadian period is compensated for repressor protein turnover rates in single cells
Gabriel CH, del Olmo MH, Widini AR, Roshanbin R, Woyde J, Hamza E, Gutu N, Zehtabian A, Ewers H, Granada AE, Herzel H, Kramer A
bioRxiv 2024.02.06.579141 Article
Gabriel CH, del Olmo MH, Widini AR, Roshanbin R, Woyde J, Hamza E, Gutu N, Zehtabian A, Ewers H, Granada AE, Herzel H, Kramer A
bioRxiv 2024.02.06.579141 Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 189980 | pDB2-hPER1-mScarlet-I-CD4-bla | Donor Vector to integrate mScarlet-I C-terminal into the human PER1 Locus to express it as a fusion protein |
| 189981 | pDB2-hPER1-Luciferase-CD4-bla | Donor Vector to integrate firefly Luciferase C-terminal into the human PER1 Locus to express it as a fusion protein |
| 189982 | pDB2-hPER1-Halo-CD4-bla | Donor Vector to integrate HaloTag C-terminal into the human PER1 Locus to express it as a fusion protein |
| 189984 | pDB2-hCRY2-mScarlet-I-CD4-bla | Donor Vector to integrate mScarlet-I C-terminal into the human CRY2 Locus to express it as a fusion protein |
| 189985 | pDB2-hCRY2-Luciferase-CD4-bla | Donor Vector to integrate firefly Luciferase C-terminal into the human CRY2 Locus to express it as a fusion protein |
| 189986 | pDB2-hCRY2-Halo-CD4-bla | Donor Vector to integrate HaloTag C-terminal into the human CRY2 Locus to express it as a fusion protein |
| 189987 | pLentiCriprV2-sgRNA-PER1-#1 | Lentiviral Cas9/sgRNA vector targeting C-terminus of hPER1, works with Addgene 189979-189982 |
| 189988 | pLentiCriprV2-sgRNA-PER1-#2 | Lentiviral Cas9/sgRNA vector targeting C-terminus of hPER1, works with Addgene 189979-189982 |
| 189989 | pLentiCriprV2-sgRNA-CRY2-#1 | Lentiviral Cas9/sgRNA vector targeting C-terminus of hCRY2, works with Addgene 189983-189986 |
| 189991 | pL6-H2B-iRFP720-bla | Lentivirial plasmid for nuclear iRFP-720 expression with blasticidin resistance |
| 189992 | pL6-H2B-iRFP720-puro | Lentivirial plasmid for nuclear iRFP-720 expression with puromycin resistance |